Sıçanlarda enteral glutamin uygulamasının intestinal iskemi-reperfüzyon hasarı üzerine etkileri
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2005
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Sağlık Bilimleri Enstitüsü
Abstract
Effects of Enteral Glutamine on Intestinal Ischemia-Reporfusion Injury in Rats Intestinal ischemia-reperfusion (l/R) injury may result in bacterial translocation in a variety of clinical conditions in surgery. This study was undertaken to assess whether oral glutamine (Gin) administration before the intestinal l/R prevents intestinal permeability in rats. The study was performed as two series having 40 female Wistar rats (200-300g) in each. Each series of animals divided into 4 groups. 1st group was untreated (received normal diet). Animals in the 2 group were only pretreated with oral glutamine (1g/kg/d for 4 days). 3rd group of rats received normal diet and underwent intestinal l/R, while the 4th group of rats was pretreated with oral glutamine in the same way and underwent intestinal l/R. An intestinal l/R model was studied by 60 minutes of superior mesenteric arterial occlusion followed by 60 minutes of reperfusion. Intestinal mucosal permeability to 51Cr EDTA was measured by collecting urine samples for a six hours period in the first series of animals. In the second series histopathological changes and plasma endotoxin levels were evaluated. Ischemia and reperfusion produced significant increases in intestinal permeability (% 2.4 ± 0.36 vs % 8.6 ± 0.61, p< 0.001), plazma endotoxin levels (0.76 ± 0.04 EU/ml vs 1.61 ± 0.19 EU/ml, p< 0.001) and worsened histopathological changes (0 vs. 3, p< 0,001) over baseline values in the untreated rats. After intestinal l/R, intestinal permeability was significantly lower in glutamine treated rats compared to those received normal diet ( % 8.6 ± 0.61 vs % 5.3 ± 0.45, p<0001) but these changes were nonsignificant in plasma endotoxin levels (1.61 ± 0.19 EU/ml vs 1.45 + 0.13 EU/ml, p= 0.48) and histopathological findings (3 vs. 2, p= 0,389). Only glutamine treatment itself did not change the intestinal permeability (% 2.4 ± 0.36 vs % 1.6 ± 2.8, p= n.s), plasma endotoxin levels ( 0.76 ± 0.04 EU/ml vs. 0.80 + 0.05 EU/ml, p= 0.59), and histopathological changes (0 vs 0, p= 0,682) significantly compared to baseline values. Although pretreatment of oral glutamine seems to be protective for intestinal mucosal integrity against l/R injury, the same effect could not be observed on histopathological changes and plasma endotoxin level alterations. Key Words: Intestinal ischemia-reperfusion, glutamine, intestinal permeability, endotoxin
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Eczacılık ve Farmakoloji