Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells

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dc.contributor.authorÇerçi, Ece
dc.contributor.authorErdost, Hatice
dc.contributor.departmentVeteriner Fakültesitr_TR
dc.date.accessioned2021-10-08T08:30:27Z
dc.date.available2021-10-08T08:30:27Z
dc.date.issued2020-06-03
dc.description.abstractThe therapeutic potential of the adipose tissue is also supported by a source of mesenchymal stem cells (MSCs), endothelial progenitor cells, mast cells, T- lymphocyte, B lymphocyte, and adipose-resident macrophages with repairing and regenerative ability. The purpose of this study was to compare adipose tissue derived mesenchymal stem cells (ADSCs) and stromal vascular fraction cells (SVF cells) in terms of the usage of non-enzymatic isolation, detection of cell adhesion, fibroblastoid cell formation, properties of cell population, cell culture duration until 3rd passages under in-vitro culture condition. We used 3 months old, 4 male Sprague Dawley rats (mean of live weight about 250 g) to examined the variation of the cell population, cell properties, phases of cellular formation, cell culturing time, subculturing duration, differences in confluency between SVF cells and ADSCs. In in-vitro expansion, ADSCs displayed higher rate of adhesiveness, homogenous cell population, faster proliferation and formation of fibroblast like cells compared to SVF cells. As a result, we showed that ADSCs have better adhesive ability, higher proliferative capacity in all of the 3 passages and require shorter time to reach confluency compared with SVF cells in vitro. These findings may contribute to future studies that deal with isolation and selection of stem cells from various tissues, as well as design clinical trials based on ADSCs and SVF cells.tr_TR
dc.identifier.endpage301tr_TR
dc.identifier.issn/e-issn1308-2817
dc.identifier.issue3tr_TR
dc.identifier.startpage295tr_TR
dc.identifier.urihttps://doi.org/10.33988/auvfd.660139tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75341
dc.identifier.volume67tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.33988/auvfd.660139tr_TR
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectAdipose tissue derived cellstr_TR
dc.subjectMesenchymal stem cellstr_TR
dc.subjectStromal vasculer fraction cellstr_TR
dc.titleNon enzymatic isolation of adipose tissue and stromal vascular fraction derived cellstr_TR
dc.typeArticletr_TR

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