Effects of Donor x Inducer Interaction on the Success of Haploid Induction and Comparison of Haploid Seed Identification Methods in the In vivo Maternal Haploid Technique in Maize
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Date
2022
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Journal ISSN
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Publisher
Ankara Üniversitesi
Abstract
This study was conducted to investigate the effect of donor and inducer
lines on the haploid induction rate in the in vivo maternal doubled haploid
technique in maize and to compare the methods used in haploid seed
separation. In the study, three donor materials were used for induction
crossing with two inducer lines. Seedling immersion and stem injection
techniques were used as chromosome doubling methods. Eye separation,
spectral measurements and image processing were used to identify seed
classes. Data were modeled using support vector machine method and
created models were evaluated over the complexity matrix. The results of
the research revealed that the haploid induction rate varies depending on
the donor genotypes and inducers, and the genotype responses against the
applied chromosome doubling method were also different. Differences
were observed in the success of the three methods compared for haploid
and diploid seed separation. The successful classification rates were 87%,
83%, and 79% in visual, spectral and image processing models,
respectively. Results showed that both spectral technique and image
processing technique can be used to distinguish haploid/diploid seeds in
in vivo maternal haploid technique.
Description
Keywords
Seed classification,, Spectral analysis,, Ploidy, Chromosome doubling