Natural Vanillin Production from Isoeugenol by Using Pseudomonas putida in Biphasic Bioconversion Medium
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Date
2022
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Publisher
Ankara Üniversitesi
Abstract
Vanillin, is one of the most demanded flavoring agents in the world.
Because of insufficient supply of natural vanillin, market demand is
usually supplied by synthetic ones. In this study, it was investigated
possibility of usage biphasic system in bioconversion of isoeugenol to
vanillin by Pseudomonas putida (HUT 8100). Organic phase was
composed of isoeugenol while biocatalyst, P. putida culture, was
dispersed in aqueous phosphate solution. Isoeugenol was used as sole
carbon source in concentrations ranging between 50-600 g L-1
. Incubation
was performed at 28 ○C, at pH 6.3 and 180 rpm shaking. Effect of initial
substrate concentration and bioconversion time were investigated.
Isoeugenol and vanillin amounts in medium were simultaneously
analyzed in HPLC system. After 120 h incubation, vanillin reached the
its highest level when 400 g L-1
isoeugenol was applied in medium. In
specified conditions, it was achieved to produce 11.95 g L-1 vanillin with
6.2% molar yield within 15 days of bioconversion. It is thought that,
obtained result by using biphasic system is very important for the
industrial applications in production of natural vanillin via bioconversion.
Description
Keywords
Bioconversion, Biphasic system, Isoeugenol