An efficient Regeneration Protocol for in vitro Direct Organogenesis in Einkorn (Triticum monococcum L.) Wheat
No Thumbnail Available
Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
Ankara Üniversitesi
Abstract
Coleoptile, leaf, and root explants of the einkorn (Triticum monococcum
ssp. monococcum) were cultured in vitro to obtain an efficient plant
regeneration protocol through direct shoot formation by using different
combinations and concentrations of various plant growth regulators. A
total of 180 different auxin and cytokinin combinations were tested for
regeneration. Shoot formation was not observed with the root and leaf
explants. Shoot formation was obtained only from the coleoptile explants,
with a mean of 1.20±0.24 shoots/explant and 86.60% of shoot formation
frequency and with a 1.20±0.53 shoots/explant and 80.00% shoot
formation frequency on medium supplemented with 0.5 mg L-1 TDZ and
1 mg L-1 TDZ plus 1 mg L-1 NAA, respectively. The shoots were
subcultured on the MS medium containing the most effective hormonal
combination concurrently continued to shoot and root formation for 45
days. It is noteworthy that 3.66±0.66 shoots per explant were induced by
MS, which contained 1 mg L-1 TDZ plus 1 mg L-1 NAA and 2.0 mg L-1
KIN plus 0.5 mg L-1 NAA for 45 days. Of the different auxin
concentrations tested for rooting, 2.0 mg L-1
IAA was predominant, with
the greatest number of roots (12.33±0.88) produced per regenerated
shoot. Finally, these well-developed plantlets were acclimatized with a
100% success rate and were transferred to the ex vitro conditions. A
highly efficient regeneration protocol for einkorn wheat was developed
using somatic tissue as an explant source for the first time.
Description
Keywords
Coleoptile, Direct regeneration protocol, Tissue culture