Phytochemical analysis of podospermum and scorzonera n-hexane extracts and the hplc quantitation of triterpenes

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dc.contributor.authorAcıkara, Özlem
dc.contributor.authorÖzbilgin, Serkan
dc.contributor.authorİşcan, Hayriye Gülçin Saltan
dc.contributor.departmentEczacılık Fakültesitr_TR
dc.date.accessioned2019-06-27T12:45:21Z
dc.date.available2019-06-27T12:45:21Z
dc.date.issued2018
dc.description.abstractPreviously tested n-hexane extracts of the Scorzonera latifolia showed promising bioactivity in vivo. Because triterpenes could account for this activity, n-hexane extracts were analyzed by HPLC to identify and quantify the triterpenes as the most abundant constituents. Other Scorzonera and Podospermum species, potentially containing triterpenic aglycones, were included in the study. An HPLC method for simultaneous determination of triterpene aglycones was therefore developed for analysis of Podospermum and Scorzonera species. n-Hexane extracts of root and aerial parts of S. latifolia, ten other Scorzonera species and two Podospermum species were studied to compare the content of triterpenes. HPLC was used for the qualitative and quantitative analysis of -amyrin, lupeol, lupeol acetate, taraxasteryl acetate, 3--hydroxy-fern-7-en-6-one acetate, urs-12-en-11-one-3-acetyl, 3--hydroxy-fern-8-en-7-one acetate, and olean-12-en-11-one-3-acetyl. Limits of detection and quantification were determined for each compound. HPLC fingerprinting of n-hexane extracts of Podospermum and Scorzonera species revealed relatively large amounts of triterpenes in a majority of investigated taxa. Lupeol, lupeol acetate, and taraxasteryl acetate were found in a majority of the species, except S. acuminata. The presence of -amyrin, 3-hydroxy-fern-7-en-6-one-acetate, urs-12-en-11-one-3-acetyl, 3-hydroxy-fern-8-en-7-one-acetate, and olean-12-en-11-one-3-acetyl was detected in varying amounts. The triterpene content could correlate with the analgesic and anti-inflammatory activity of Scorzonera, which was previously observed and Scorzonera species that have been determined to contain triterpenes in large amounts and have not yet been tested for their analgesic activity should be tested for their potential analgesic and anti-inflammatory potential. The presented HPLC method can be used for analysis of triterpene aglycones, for example dedicated to chemosystematic studies of the Scorzonerinae.tr_TR
dc.description.indexScopus
dc.description.indexWos
dc.description.indexPubmed
dc.identifier.endpage12tr_TR
dc.identifier.issue07tr_TR
dc.identifier.other10.3390/molecules23071813
dc.identifier.startpage01tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/66838
dc.identifier.volume23tr_TR
dc.language.isoentr_TR
dc.relation.journalMoleculestr_TR
dc.subjectHPLCtr_TR
dc.subjectPodospermumtr_TR
dc.subjectScorzoneratr_TR
dc.subjectTriterpenestr_TR
dc.titlePhytochemical analysis of podospermum and scorzonera n-hexane extracts and the hplc quantitation of triterpenestr_TR
dc.typeArticletr_TR

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